- #Agilent 3000 micro gc 4 channel software portable#
- #Agilent 3000 micro gc 4 channel software software#
- #Agilent 3000 micro gc 4 channel software plus#
The B term considers band broadening due to longitudinal diffusion, and the C term takes into consideration the band broadening due to resistance to mass transfer in the stationary and mobile phases. This effect is known as “eddy” diffusion this effect becomes more dominant as the particle size of the packing material increases. In this equation, A accounts for band broadening due to multiple path lengths traveled by the solute molecules through a packed column. In the simplified form, the van Deemter equation can be written as (2)where u̅ is the average linear velocity of the mobile phase. The theory has undergone several modifications since then. (36) made a significant attempt to explain the mechanism of band broadening by using a rate theory and correlated H to a number of different independent sources of sample dispersion. The value of H is calculated by dividing the column length ( L) by the plate number ( N). Alternatively, the separation efficiency of a column can be expressed in terms of the length of the column that corresponds to one theoretical plate, which is known as height equivalent to a theoretical plate (HETP) or simply plate height ( H). The number of theoretical plates increases with the increase in the column length therefore, chromatographers express the average performance of a column by reporting the number of theoretical plates per meter of column length. Additionally, temperature programming would vastly overestimate the values of plate numbers.
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(35) Typically, solutes with low values of retention factor exhibit higher plate numbers. The number of theoretical plates is experimentally determined under isothermal conditions using a test solute with a retention factor more than 5. The narrower the peak width is, the greater is the plate number and separation efficiency.
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The peak width determines the chromatographic efficiency, which is expressed in terms of the number of theoretical plates or plate number ( N). Ideally, the separation process results in the generation of a Gaussian-shaped peak. Additionally, extra column effects, such as injection pulse width and dead volumes, also contribute to the dispersion. Even identical solute molecules, owing to the randomness of different processes, reach the detector at different times leading to a distribution.
#Agilent 3000 micro gc 4 channel software software#
A μGC comprises a number of components, including a source of carrier gas, preconcentrator-injector, separation column, detector, pump, valves, and software for instrument control, data acquisition, and analysis.Īs the components of a mixture move through a separation column, they undergo a number of processes, and hence the components exit the column at different times.
#Agilent 3000 micro gc 4 channel software portable#
(19,25,26) Throughout this article, we use the term “micro gas chromatograph” (μGC) to refer any field portable versions of a GC comprising one or more microfabricated components. Moreover, the use of the term “micro” is also not based on the type of column used, as miniature GC systems comprising conventional capillary columns are sometimes named as micro GCs.
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#Agilent 3000 micro gc 4 channel software plus#
For example, INFICON 3000 Micro GC (portable model) weighs 36.5 lb, Agilent 490 Micro GC (with 4 channels) weighs 23.4 lb, and Vernier Mini GC Plus (developed by Seacoast Science, Inc.) weighs 2.87 lb, indicating that the use of word “mini” or “micro” is not based on weights. (20−24) The nomenclature of these miniaturized GC-based devices is not clear-cut. There has been increased marketability of portable GC instruments, and these products are being developed in both research (5−19) and commercial laboratories (Table S1 in the Supporting Information). With an aim to develop compact, low power, and field portable GC instruments, considerable research has been conducted over the past 40 years. (4) Hence, these instruments are normally not field portable.
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While a conventional GC is a powerful and versatile tool, it is relatively bulky and requires high power with a typical peak power requirement of 2000–3000 V-ampere. Among a number of portable devices, a miniaturized version of gas chromatography (GC) system is a very promising technique for rapid and sensitive analysis of complex chemical mixtures.